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Image Search Results
Journal: Circulation
Article Title: FTO-Dependent m6A Regulates Cardiac Function During Remodeling and Repair
doi: 10.1161/CIRCULATIONAHA.118.033794
Figure Lengend Snippet: Quantification of m6A in total or polyA+ RNA in LV of A, human, n=6–11; from infarct/peri-infarct areain B, pig, n=3–6, C, mouse, n=5–11; from non-infarct area in D, pig, n=3 and E, mouse, n=3–6. Error bars represent SEM. *P<0.05, **P<0.01, ***P<0.001, compared with non-failing or sham. n.s., non significant.
Article Snippet: Column purified total RNA (miRNeasy, Qiagen) or
Techniques:
Journal: Circulation
Article Title: FTO-Dependent m6A Regulates Cardiac Function During Remodeling and Repair
doi: 10.1161/CIRCULATIONAHA.118.033794
Figure Lengend Snippet: A,quantification of mRNA, n=3–6, B, representative immunoblots, C, densitometry quantification of protein, n=5–8 for m6A regulators in human non-failing and failing hearts. D, quantification of mRNA, n=3–7, E, representative immunoblots and F, densitometry quantification of protein, n=3–4 at different time points in mouse LV. G, qRT-PCR quantification of selected mRNA expressions in mouse LV, n=4–8. mRNA/protein data represented as F/MI normalized to NF/sham. Error bars represent SEM. *P<0.05, **P<0.01, ***P<0.001, compared with non-failing or sham.
Article Snippet: Column purified total RNA (miRNeasy, Qiagen) or
Techniques: Western Blot, Quantitative RT-PCR
Journal: Circulation
Article Title: FTO-Dependent m6A Regulates Cardiac Function During Remodeling and Repair
doi: 10.1161/CIRCULATIONAHA.118.033794
Figure Lengend Snippet: Quantification of A, Fto mRNA, n=3–4, B, m6A in total RNA, n=3–5, C, cells with arrhythmic events, n=47–90 cells per group from 4–12 rats. D, Representative Ca2+ transients obtained from pacing-induced myocytes. Measurements of E, maximal Ca2+ amplitude, F, time to 50% decay, G, Tau, H, cell shortening, n=23–74 cells per group from 4–12 rats. Sarcomere and Ca2+ transients were recorded at 1Hz pacing stimulation frequency with MyoPacer Field Stimulator (IonOptix MA, USA). Abbreviations, Unt: untreated; siCtrl: siRNA control, siFto: siRNA-mediated Fto knockdown, adnull: adenovirus with empty CMV promoter, adFto: adenovirus with full length Fto. Error bars represent SEM. *P<0.05, **P<0.01, ***P<0.001by one-way ANOVA.
Article Snippet: Column purified total RNA (miRNeasy, Qiagen) or
Techniques:
Journal: Circulation
Article Title: FTO-Dependent m6A Regulates Cardiac Function During Remodeling and Repair
doi: 10.1161/CIRCULATIONAHA.118.033794
Figure Lengend Snippet: A, human MeRIP-qPCR showing m6A enrichment in mRNA. B,FTO mRNA and C, SERCA2A mRNA, n=3 expressions in AC16 human myocytes. Fto mRNA expression in D, aavFto-MI and E, adFto mice 4w post-MI, n=3–5. F, Representative immunoblots showing Serca2a protein expression at 4w post-MI. Error bars represent SEM. *P<0.05, ***P<0.001. A-C, student t-test; D and E, one-way ANOVA.
Article Snippet: Column purified total RNA (miRNeasy, Qiagen) or
Techniques: Expressing, Western Blot